WEI Zhaohui,WAN Shengfang,SHU Chang,et al.Effects of Hedysarum Polybotrys Polysacchcaide on Expressions of c-kit, Cx43 Gene and Protein in Small Intestine of Diabetic Gastroparesis Rats[J].zhongguo zhongyiyao xinxi zazhi,2020,27(8):46-51.[doi:10.3969/j.issn.1005-5304.202001159]
红芪多糖对糖尿病胃轻瘫大鼠小肠组织c-kit、Cx43基因和蛋白表达的影响
- Title:
- Effects of Hedysarum Polybotrys Polysacchcaide on Expressions of c-kit, Cx43 Gene and Protein in Small Intestine of Diabetic Gastroparesis Rats
- 文章编号:
- 1005-5304(2020)08-0046-05
- Keywords:
- diabetic gastroparesis ; Hedysarum polybotrys polysacchcaide; c-kit ; Cx43 ; rats
- 分类号:
- R285.5
- 文献标志码:
- A
- 摘要:
- 目的 观察红芪多糖（HPS）对糖尿病胃轻瘫（DGP）大鼠小肠组织酪氨酸激酶受体c-kit、缝隙连接蛋白Cx43基因和蛋白表达的影响，探讨其对DGP大鼠肠动力障碍的作用机制。方法 采用一次性大剂量腹腔注射链脲佐菌素联合高糖高脂饲料不规则喂养方式复制DGP模型，将72只Wistar雄性大鼠随机分为空白组、模型组、阳性对照组和HPS高、中、低剂量组，每组12只。阳性对照组予莫沙必利药液0.7 g/（kg?d）灌胃，HPS高、中、低剂量组分别予HPS药液0.2、0.1、0.05 g/（kg?d）灌胃，空白组和模型组分别予等体积纯净水灌胃，每日1次，连续8周。HE染色观察小肠病理学结构改变；RT-PCR和Western blot分别检测小肠组织c-kit、Cx43 mRNA和蛋白的表达。结果 HE染色结果显示，模型组大鼠小肠正常结构被破坏，大量炎性细胞浸润；各给药组大鼠情况改善，可见正常肠绒毛、中央乳糜管等结构，炎性细胞浸润减少，其中以HPS高剂量组改善明显。与空白组比较，模型组大鼠小肠组织c-kit、Cx43 mRNA和蛋白表达显著降低（P＜0.01）；与模型组比较，各给药组大鼠小肠组织c-kit、Cx43 mRNA和蛋白表达显著升高（P＜0.05，P＜0.01），HPS高剂量组作用最显著。结论 HPS通过上调c-kit、Cx43 mRNA和蛋白的表达提高小肠动力，改善DGP模型大鼠肠动力障碍。
- Abstract:
- Objective To observe the effects of Hedysarum polybotrys polysacchcaide (HPS) on the expressions of tyrosine kinase receptor c-kit , gap junction protein Cx43 and protein in the small intestine of diabetic gastroparesis (DGP) rats; To investigate the mechanism of HPS on intestinal motility disorders in DGP rats. Methods The DGP model was duplicated by a single high-dose intraperitoneal injection of STZ combined with high-sugar and high-fat diets. 72 Wistar male rats were randomly divided into blank group, model group, positive control group, HPS high-, medium-, and low-dosage groups, with 12 rats in each group. The positive control group was administrated with 0.7 g/(kg?d) of Mosabilli, and HPS high-, medium-, and low-dosage groups were administered with HPS at 0.2, 0.1, 0.05 g/(kg?d), respectively. Blank group and model group were given equal volume of pure water for gavage, once a day for 8 weeks continuously. The pathological structure of the small intestine was observed by HE staining; the expressions of c-kit, Cx43 mRNA and protein in the small intestine were detected by RT-PCR and Western blot, respectively. Results HE staining results showed that the normal structure of the small intestine in the model group was destroyed, and a large number of inflammatory cells were infiltrated. After administration, the condition of the rats in each administration group improved, showing that the inflammatory cell infiltration was reduced compared with the normal intact villi and central lacteal ducts and other structures; the HPS high-dosage group improved significantly. Compared with the blank group, the expressions of c-kit, Cx43 mRNA and protein in the small intestine tissue of the model group rats were significantly reduced ( P<0.01). Compared with the model group, the expressions of c-kit, Cx43 mRNA and protein in the small intestine of rats in each administration group significantly increased (P<0.05，P<0.01), and HPS high-dosage group showed the most obvious effects. Conclusion HPS can increase the expressions of c-kit, Cx43 mRNA and protein of small intestine motility and improve the intestinal motility of DGP model rats.
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备注/Memo
基金项目：国家自然科学基金（81560718）；甘肃省中医药管理局科研课题（GZK-2017-3） 通讯作者：万生芳，E-mail：wanshengfang@163.com
更新日期/Last Update:
2020-07-20