WANG Xu,SUN Nina,TIAN Yuefeng,et al.Effects of Different Types of Moxibustion on Expressions of PD-1 and PD-L1 mRNA in Immunosuppressive Rabbits Induced by C yclophosphamide[J].zhongguo zhongyiyao xinxi zazhi,2020,27(11):66-70.[doi:10.19879/j.cnki.1005-5304.202007162]
不同灸法对环磷酰胺所致免疫抑制兔脾组织程序性死亡因子-1及其配体表达的影响
- Title:
- Effects of Different Types of Moxibustion on Expressions of PD-1 and PD-L1 mRNA in Immunosuppressive Rabbits Induced by C yclophosphamide
- 文章编号:
- 1005-5304(2020)11-0066-05
- Keywords:
- moxibustion ; PD-1 ; PD-L1 ; cyclophosphamide ; immunosuppressive ; rabbits
- 分类号:
- R245
- 文献标志码:
- A
- 摘要:
- 目的 观察不同灸法对环磷酰胺所致免疫抑制兔脾组织程序性死亡因子-1（PD-1）及其配体PD-L1表达的影响。方法 大耳白兔32只随机分为空白组、模型组、隔药饼灸组、艾灸组。除空白组外，其余3组腹腔注射环磷酰胺制备免疫抑制兔模型，连续7 d。成模后，隔药饼灸组和艾灸组分别给予隔药饼灸、艾灸干预，隔日灸，共灸10次。治疗结束后次日动物麻醉，摘取脾脏。采用RT-qPCR检测PD-1、PD-L1 mRNA表达，免疫组化检测PD-1蛋白表达。结果 与空白组比较，模型组兔脾组织PD-1、PD-L1 mRNA表达明显升高（P＜0.01）；与模型组比较，隔药饼灸组兔脾组织PD-1、PD-L1 mRNA表达明显降低（P＜0.01），艾灸组兔脾组织PD-L1 mRNA表达明显降低（P＜0.05），PD-1 mRNA表达虽有降低趋势但差异无统计学意义（P＞0.05）。免疫组化结果显示，兔脾组织棕褐色PD-1阳性表达主要在细胞浆、细胞膜或间质，与空白组比较，模型组兔脾组织PD-1蛋白表达显著升高（P＜0.01）；与模型组比较，隔药饼灸组和艾灸组兔脾组织PD-1蛋白表达显著降低（P＜0.01，P＜0.05）。结论 不同灸法对免疫抑制兔免疫功能均有明显改善作用，隔药饼灸较艾灸更具优势。
- Abstract:
- Objective To observe the changes of expressions of PD-1 and PD-L1 in immunosuppressive rabbits induced by cyclophosphamide through different types of moxibustion. Methods Totally thirty-two rabbits were randomly divided into blank group , model group , herbal cake-separated moxibustion group and moxibustion group. Except the blank group, the other three groups were intraperitoneally injected with cyclophosphamide to make immunosuppressive rabbit model for 7 days. After the model was established , herbal cake-separated moxibustion group and moxibustion group were treated with herbal cake-separated moxibustion and directed-moxibustion respectively , once every other day , 10 times in total. The next day after the treatment , the animals were anesthetized and the spleen was removed. The expressions of PD-1 and PD-L1 mRNA were detected by real-time fluorescence quantitative PCR , and the expression of PD-1 protein was detected by immunohistochemistry. Results Compared with the blank group, the expressions of PD-1 and PD-L1 mRNA in the spleen tissue of the model group increased significantly (P<0.01). Compared with the model group, the expressions of PD-1 and PD-L1 mRNA in the spleen tissue of the rabbits in the herbal cake-separated moxibustion group was significantly lower (P<0.01), and the expression of PD-L1 mRNA in the spleen tissue of the moxibustion group was significantly lower (P<0.05), while the PD-1 mRNA showed a decreasing trend , without statistical significance (P>0.05). Immunohistochemical results showed that the positive expression of PD-1 in rabbit spleen tissue was mainly in the cytoplasm, cell membrane or interstitial. Compared with the blank group , the expression of PD-1 protein in the rabbit spleen tiss ue of the model group significantly increased (P<0.01). Compared with the model group, the expression of PD-1 protein in the spleen tissues of rabbits in the herbal cake-separated moxibustion group and moxibustion group was significantly reduced (P<0.01, P<0.05). Conclusion Different types of moxibustion have obviously improved the immune function of immunosuppressive rabbits , and herbal cake-separated moxibustion has more advantages than moxa moxibustion.
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备注/Memo
基金项目：国家自然科学基金（81674062）
更新日期/Last Update:
2020-10-19