[1]玛依努尔·艾力,哈木拉提·吾甫尔.维吾尔药异常黑胆质成熟剂总黄酮逆转肝癌细胞耐药性的作用[J].中国中医药信息杂志,2007,14(9):31-33.
　Mayinur,Hamulati.The Reverse Effect of Uygur Medicine ASMq on the Drug Resistance Cells of Liver Cancer[J].zhongguo zhongyiyao xinxi zazhi,2007,14(9):31-33.
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维吾尔药异常黑胆质成熟剂总黄酮逆转肝癌细胞耐药性的作用

《中国中医药信息杂志》[ISSN:1005-5304/CN:11-3519/R] 卷: 14卷 期数: 2007年9期 页码: 31-33 栏目: 实验研究 出版日期: 2007-09-15
Title:
The Reverse Effect of Uygur Medicine ASMq on the Drug Resistance Cells of Liver Cancer
文章编号:
1005-5304(2007)09-0031-03
作者:
玛依努尔·艾力1; 哈木拉提·吾甫尔2
1.新疆医科大学第一附属医院,新疆 乌鲁木齐 830054；2.新疆医科大学,新疆 乌鲁木齐 830000
Author(s):
Mayinur1;  Hamulati2
1.The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China；2.Xinjiang Medical University, Urumqi 830000, China
关键词:
维吾尔药; 异常黑胆质成熟剂总黄酮; 肝癌; 多药耐药性
Keywords:
uygur traditional medicine; ASMq; liver cancer; multi-drug resistance
分类号:
R291.5
文献标志码:
A
摘要:
目的 研究维吾尔药异常黑胆质成熟剂总黄酮(ASMq)体外对肝癌耐药细胞株 Bel/Fu逆转肿瘤耐药性的作用。方法 应用MTT法测定应用ASMq前后5-Fu对Bel/Fu耐药细胞株的IC50,计算其逆转倍数；浓度依赖试验确定ASMq逆转浓度；耐药性的集落形成实验初步确定ASMq 逆转Bel/Fu的作用。结果 ASMq对Bel/Fu细胞 IC50 是2 000.68 μg/mL,其浓度为390 μg/mL时对细胞无明显的杀伤作用,逆转倍数为7.8,与抗癌药物5-Fu有协同作用,CDI为0.89。集落形成实验从形态上证实ASMq逆转MDR 作用,随ASMq浓度的加大集落逐渐变小。结论 ASMq对肝癌耐药细胞株Bel/Fu细胞多药耐药有逆转作用。
Abstract:
Objective To investigate the reverse effect of the extracted solution of uygur medicine ASMq on the liver cancer drug-resistance cell line Bel/Fu in vitro. Methods MTT assay was used to determine the IC50 of 5-Fu on Bel/Fu cell line and the reverse multiples of the drug-resistance before and after ASMq treatment. The concentration was used to determine the fold reversal of ASMq. By clone forming text to determine the reversal effect on drug resistance of Bel/Fu cell line. Results The IC50 value of ASMq crude extract was 2 000.68 μg/mL. ASMq at 390 μg/mL concentration was no toxic but had a coordinate effect on Bel/Fu cell line with 5-Fu. The fold reversal of ASMq combining with 5-Fu was 7.8, CDI was 0.89. The clone forming size became smaller comply with the concentration of ASMq. Conclusion ASMq has reversal effect on drug resistance of Bel/Fu cell line.

参考文献/References:

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[3] 田 晖,潘启超.双苄基异喹啉生物碱粉防异碱与小檗胺逆转多药耐药性的比较研究[J].药学学报,1997,32：245.

[4] Abdiryim Y,Halmurat U, Umar A,et al.Protective effects of Munziq and Mushil of Abnormal Savda to mitochondrial oxidative damage[J].Fundam Clin Pharmacol,2004,18(4)：471－476.

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备注/Memo

基金项目：全国高等学校科技创新工程重大项目培育资金项目(706058)

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