双黄清散剂质量标准研究

Title:

Study on Quality Standard for Shuanghuangqing Powder

文章编号:

1005-5304(2010)10-0051-03

作者:

刘光斌; 张迅; 刘效栓; 张宇博

酒钢医院,甘肃 嘉峪关 735100

Author(s):

LIU Guang-bin;  ZHANG Xun;  LIU Xiao-shuan;  et al

Jiugang Hospital, Jiayuguan 735100, China

关键词:

双黄清散剂; 高效液相色谱法; 薄层色谱法; 盐酸小檗碱; 质量标准

Keywords:

Shuanghuangqing Powder; HPLC; TLC; berberine hydrochloride; quality standard

分类号:

R284.1

DOI:

10.3969/j.issn.1005-5304.2010.10.022

文献标志码:

A

摘要:

目的 建立双黄清散剂的质量标准。方法 采用薄层色谱法鉴别人工牛黄、黄柏、青黛、甘草、龙胆草；采用高效液相色谱法测定黄柏中有效成分盐酸小檗碱的含量。结果 薄层鉴别方法专属性强,重复性好,空白无干扰；盐酸小檗碱在0.080 2～0.802 ?g范围内呈良好的线性关系,r＝0.999 7,平均加样回收率为98.52%,RSD＝1.05%(n＝9)。结论 本研究中建立的定性定量方法操作简便,专属性强,准确可靠,可以有效控制双黄清散剂的质量。

Abstract:

Objective To establish the quality standard of Shuanghuangqing Powder. Methods Artificial Calculus Bovis, Cortex Phellodendri Chinensis, Indigo Naturalis, Radix Glycyrrhizae, Radix Gentianae in Shuanghuangqing Powder were identified by TLC, and content of berberine hydrochloride was determined by HPLC. Results The method for identification had good specificity and repeatability. There was no interference in blank control. The berberine hydrochloride determination method had good linearity from 0.080 2 to 0.802 ?g (r＝0.999 7). The average recovery was 98.52% and the relative standard deviation (RSD) was 1.05% (n＝9). Conclusion The qualitative and quantitative method is simple, specific, accurate and reliable. It can be used to effectively control the quality of Shuanghuangqing Powder.