[1]尚红霞,孙蔚明,程卫东,等.红芪多糖对非酒精性脂肪肝大鼠脂代谢及硬脂酰辅酶A去饱和酶1基因表达的影响[J].中国中医药信息杂志,2014,21(7):47-49.[doi:10.3969/j.issn.1005-5304.2014.07.014]
　SHANG Hong-xia,SUN Wei-ming,CHENG Wei-dong,et al.Effects of Hedysarum Polybotys Saccharide on Lipid Metabolism and Expression of Stearoyl-CoA Desaturase-1 Gene in NAFLD Rats[J].zhongguo zhongyiyao xinxi zazhi,2014,21(7):47-49.[doi:10.3969/j.issn.1005-5304.2014.07.014]

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红芪多糖对非酒精性脂肪肝大鼠脂代谢及硬脂酰辅酶A去饱和酶1基因表达的影响

《中国中医药信息杂志》[ISSN:1005-5304/CN:11-3519/R] 卷: 21卷期数: 2014年7期页码: 47-49 栏目: 实验研究出版日期: 2014-06-26

Title:: Effects of Hedysarum Polybotys Saccharide on Lipid Metabolism and Expression of Stearoyl-CoA Desaturase-1 Gene in NAFLD Rats

文章编号:: 1005-5304(2014)07-0047-03

作者:: 尚红霞¹; 孙蔚明²; 3; 程卫东²; 4; 段永强¹; 2; 孙晓芬²; 1.甘肃中医学院,甘肃兰州 730020；2.兰州大学基础医学院中西医结合研究所,甘肃兰州 730020；3.兰州大学第一医院,甘肃兰州 730020；4.南方医科大学中医药学院,广东广州 510515

Author(s):: SHANG Hong-xia¹; SUN Wei-ming²; 3; CHENG Wei-dong²; 4; DUAN Yong-qiang¹; 2; SUN Xiao-fen²; 1.Gansu College of TCM, Lanzhou 730020, China；2.Institute of Integrated Chinese and Western Medicine, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730020, China；3.The First Hospital of Lanzhou University, Lanzhou 730020, China；4.School of TCM, Southern Medical University, Guangzhou 510515, China

关键词:: 红芪多糖; 非酒精性脂肪肝; 脂代谢; 硬脂酰辅酶A去饱和酶1; 大鼠

Keywords:: hedysarum polybotys saccharide; NAFLD; lipid metabolism; SCD-1; rats

分类号:: R285.5

DOI:: 10.3969/j.issn.1005-5304.2014.07.014

文献标志码:: A

摘要:: 目的观察红芪多糖对非酒精性脂肪肝(NAFLD)大鼠脂代谢及调控基因硬脂酰辅酶A去饱和酶1 (SCD-1)表达的影响,探讨其对NAFLD的干预效应。方法受试动物按随机数字表法分为空白组和实验组,实验组采用高脂饲料喂养8周造模,随机分为模型组、阳性对照组、红芪多糖组,药物干预8周后分别检测各组大鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)水平及三酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-c)、低密度脂蛋白胆固醇(LDL-c)含量,采用半定量PCR检测肝脏SCD-1基因表达。结果与空白组比较,模型组大鼠血清ALT、AST和LDL-c、TC、TG升高(P＜0.05,P＜0.01),HDL-c降低(P＜0.01)；SCD-1基因表达降低(P＜0.01)；与模型组比较,红芪多糖组血清ALT、AST降低(P＜0.01),LDL-c、TC、TG降低(P＜0.05,P＜0.01),HDL-c升高(P＜0.01)；肝组织SCD-1基因表达升高(P＜0.01)。结论红芪多糖具有调节NAFLD大鼠脂代谢紊乱和促进调控基因SCD-1表达的作用。

Abstract:: Objective To investigate the effects of hedysarum polybotys saccharide (HPS) on lipid metabolism and the expression of stearoyl-CoA desaturase-1(SCD-1) gene in rats with non-alcoholic fatty liver disease (NAFLD). To discuss the interfering effects of HPS on NAFLD. Methods The SD rats were randomly divided into the blank control group and the experiment group. Rats in the experiment group were fed with lipid rich food for 8 weeks to establish model and were randomly divided into model group, drug positive group and HPS group. After 8 weeks of drug intervention, the level of ALT, AST, TC, TG, HDL-c and LDL-c were measured with automatic chemistry analyzer, and expression of SCD-1 gene was measured by semi-quantitative polymerase chain reaction. Results Compared with blank control group, serum ALT, AST and TC, TG, LDL-c of model group were higher (P＜0.05, P＜0.01), the level of HDL-c of model group and the expression of SCD-1 gene were lower (P＜0.01). Compared with model group, HPS was useful to decrease serum ALT, AST, LDL-c, TC and TG (P＜0.05, P＜0.01), and increase the level of HDL-c (P＜0.01) and the expression of SCD-1 gene (P＜0.01). Conclusion HPS had a positive effect on regulating lipid metabolic disturbance of NAFLD rats and promoting the expression of regulatory gene SCD-1.

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备注/Memo

基金项目：甘肃省中医药管理局资助项目(GZK-2012-65)

更新日期/Last Update:

2014-06-12