ZHANG Hua-zhi,JIN Zhi-sheng,LIU Ying,et al.Effects of Hedysari Polysaccharide on Expressions of TSP-1 and PDGF-B in Retina of Diabetic Rats[J].zhongguo zhongyiyao xinxi zazhi,2017,24(3):38-42.[doi:10.3969/j.issn.1005-5304.2017.03.010]
红芪多糖对糖尿病大鼠视网膜血小板反应蛋白-1和血小板源性生长因子-B表达的影响
- Title:
- Effects of Hedysari Polysaccharide on Expressions of TSP-1 and PDGF-B in Retina of Diabetic Rats
- 文章编号:
- 1005-5304(2017)03-0038-05
- Keywords:
- diabetes mellitus; retina; Hedysari Polysaccharide; TSP-1; PDGF-B; rats
- 分类号:
- R285.5
- 文献标志码:
- A
- 摘要:
- 目的 观察红芪多糖（HPS）对糖尿病大鼠视网膜血小板反应蛋白-1（TSP-1）和血小板源性生长因子-B（PDGF-B）表达的影响，探讨其对糖尿病视网膜病变的保护作用及可能机制。方法 采用链脲佐菌素腹腔注射建立糖尿病模型。雄性Wistar大鼠随机分为模型组、多贝斯组和HPS高、中、低剂量组，另设正常组，每组10只。各给药组给予相应药物灌胃，模型组和正常组给予等量生理盐水灌胃，1次/d，连续8周。qRT-PCR和免疫组化检测TSP-1和PDGF-B mRNA和蛋白表达；HE染色镜下观察视网膜的结构。结果 模型组视网膜各层结构清晰、完整，但外核层疏松变薄、排列紊乱，神经节细胞数量稍减少；各给药组较模型组明显好转。与正常组比较，模型组视网膜TSP-1 mRNA和蛋白表达明显降低（P＜0.01），PDGF-B mRNA和蛋白表达明显升高（P＜0.01）；与模型组比较，各给药组TSP-1 mRNA和蛋白表达明显升高（P＜0.05，P＜0.01），PDGF-B mRNA和蛋白表达明显降低（P＜0.01）；HPS高剂量组与其余给药组比较，TSP-1和PDGF-B mRNA和蛋白表达差异有统计学意义（P＜0.05，P＜0.01）。结论 HPS可能通过升高糖尿病大鼠视网膜组织TSP-1的表达和降低PDGF-B的表达来阻遏糖尿病视网膜病变进程中新生血管生成及增殖，从而起到保护视网膜的作用。
- Abstract:
- Objective To observe the effects of Hedysari Polysaccharide (HPS) on the expressions of TSP-1 and PDGF-B in the retina of diabetic rats; To discuss the protective effect and possible mechanism on diabetic retinopathy. Methods The diabetic model was established by intraperitoneal injection of streptozotocin. 50 male SPF Wistar rats were randomly divided into 5 groups: model group, calcium dobesilate group, and HPS high-, medium-, and low-dose group, extra 10 rats were set as the normal group, 10 rats in each group. Each administration group was given relevant medicine for gavage, while model group and normal control group were given same amount NS for gavage, once a day for 8 weeks. The mRNA and protein expression of TSP-1 and PDGF-B were detected by qRT-PCR and immunohistochemistry. The retinal structure was observed by HE staining. Results HE staining showed that each layer of the retina of the model group was clear and complete, but the outer nucleus layer became looser, thinner and more disorderly, and the number of ganglion cells decreased slightly; the administration groups were improved markedly compared with the model group. Compared with the normal control group, the mRNA level and protein expression of retina TSP-1 on the model group dramatically dropped (P<0.01), and those of PDGF-B strikingly increased (P<0.01); Compared with the model group, the mRNA level and protein expression of retina TSP-1 on all administration groups rose (P<0.05, P<0.01), and those of PDGF-B went down (P<0.01); Compared with all other administration groups, there was statistical significance in the mRNA level and protein expression of retina TSP-1 and PDGF-B on HPS high-dose group (P<0.05, P<0.01). Conclusion HPS may prevent the angiogenesis and proliferation in diabetic retinopathy process through adjusting the content of TSP-1 and PDGF-B in retina of diabetic rats so as to protect the retina.
参考文献/References:
[1] NENTWCH M M, ULBIG M W. Diabetic retinopathy-ocular complications of diabetes mellitus[J]. World J Diabetes,2015,6(3)：489-499. [2] YAU J W, ROGERS S L, KAWASAKI R, et al. Global prevalence and major risk factors of diabetic retinopathy[J]. Diabetes Care, 2012,35(3)：556-564. [3] REN G, FUSE N, ABE T, et al. mRNA expression of proto-oncogenes and platelet-derived growth factor in proliferative vitreoretinal diseases[J]. Jpn J Ophthalmol,2000,44(3)：308-311. [4] ZEHETNER C, KIRCHMAIR R, NEURURER S B, et al. Systemic upregulation of PDGF-B in patients with neovascular AMD[J]. Invest Ophthalmol Vis Sci,2014,55(1)：337-344. [5] KONG P, CAVALERA M, FRANGOGIANIS N G. The role of thrombospondin (TSP)-1 in obesity and diabetes [J]. Adipocyte,2014,3(1)：81-84. [6] SHEN J, VIL M D, ZHANG H, et al. An antibody directed against PDGF receptor beta enhances the antitumor and the anti-angiogenic activities of an anti-VEGF receptor 2 antibody[J]. Biochem Biophys Res Commun,2007,357(4)：1142-1147. [7] 刘丽娟,李茂欣,汤旭磊,等.糖尿病大鼠视网膜早期病变中PDGF-B的表达[J].第四军医大学学报,2005,26(23)：2159-2161. [8] 汝亚琴,金智生,张花治,等.红芪多糖对db/db小鼠视网膜血管内皮生长因子表达的影响[J].兰州大学学报：医学版,2015,41(4)：15-19. [9] 张花治,刘莹,李桂珍,等.红芪多糖对糖尿病大鼠视网膜VEGF和Ets-1表达的影响[J].中医研究,2016,29(2)：60-63. [10] KOYAMA N, WATANABE S, TEZUKA M, et al. Migratory and proliferative effect of platelet-derived growth factor in rabbit retinal endothelial cells： evidence of an autocrine pathway of platelet-derived growth factor[J]. Journal of Cellular Physiology,1994,158(1)：1-6. [11] 魏玉娇,金智生,朱真灵,等.红芪多糖对糖尿病肾病db/db小鼠肾脏保护作用及其对肾组织PKCα与VEGF表达的影响[J].北京中医药大学学报,2014,37(2)：116-120. [12] 金智生,朱真灵,魏玉娇,等.红芪多糖对糖尿病肾病db/db小鼠肾功能和肾脏组织中GluT-1 mRNA和蛋白表达水平的影响[J].吉林大学学报：医学版,2014,40(3)：519-524. [13] 金智生,魏玉娇,朱真灵,等.红芪多糖对db/db小鼠肾组织血管内皮生长因子、色素上皮细胞衍生因子表达的影响[J].中医杂志,2014, 55(15)：1327-1330. [14] 祁雪艳,金智生,陈长浩,等.红芪多糖对早期糖尿病肾病db/db小鼠肾脏保护作用及PKC/TIMP-1表达的影响[J].中国老年学杂志,2015, 31(7)：5053-5056.
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备注/Memo
基金项目：国家自然科学基金地区基金(81360538)；甘肃省青年科技基金计划（145RJYA297）
更新日期/Last Update:
2017-02-15