ZHAO Mei-yu,SONG Liang-ke,REN Yao-yao,et al.Content Determination of Three Alkaloids in Aconitum Pendulum and Habitually Medicinal Materials[J].zhongguo zhongyiyao xinxi zazhi,2018,25(7):83-86.[doi:10.3969/j.issn.1005-5304.2018.07.020]
藏药铁棒锤及其习用品中3种生物碱含量测定
- Title:
- Content Determination of Three Alkaloids in Aconitum Pendulum and Habitually Medicinal Materials
- 文章编号:
- 1005-5304(2018)07-0083-04
- 分类号:
- R284.1
- 文献标志码:
- A
- 摘要:
- 目的 建立同时测定铁棒锤及其习用品中苯甲酰乌头碱、乌头碱、3-脱氧乌头碱的HPLC含量测定方法，为铁棒锤药材质量标准毒性成分控制及其与习用品区别提供依据。方法 采用Agilent Eclipse XDB-C18色谱柱（250 mm×4.6 mm，5 μm），流动相为乙腈-0.04%三乙胺（每1000 mL水加4 mL三乙胺、1.68 mL磷酸），梯度洗脱，检测波长235 nm，流速1 mL/min，进样量10 μL，柱温25 ℃。结果 苯甲酰乌头碱、乌头碱、3-脱氧乌头碱分离度良好，3种成分在检测范围内均呈良好线性（r＞0.999），平均加样回收率为97.66%～98.47%，RSD为0.84%～1.60%。结论 铁棒锤及其习用品中3种生物碱的含量差异较大，多裂乌头A. polyschistum、缩梗乌头A. sessiliflorum应另立药名加以区别。
- Abstract:
- Objective To establish a method for determining benzoylaconine, aconitine and 3-deoxyaconitine in Aconitum pendulum and the habitually medicinal materials by HPLC ; To provide basis for control of quality standard toxicity composition in Aconitum pendulum and difference between Aconitum pendulum and the habitually medicinal materials. Methods Agilent Eclipse XDB-C18 column (250 mm × 4.6 mm ，5 μm) was used at 25 ℃ with the mobile phase consisting of acetonitrile-0.04% trimethylamine (each 1000 mL of water plu s 4 mL triethylamine and 1.68 mL phosphoric acid) by gradient elution; detection wavelength was 235 nm ; the flow rate was 1 mL/min; injection volume was 10 μL. Results The benzoylaconine, aconitine and 3-deoxyaconitine had good separation and linear relationship in the corresponding range ( r>0.999). The average recovery rates were 97.66%–98.47%, and RSD were 0.84%–1.60%. Conclusion The contents of 3 alkaloid were different in Aconitum pendulum and the habitually medicinal materials. Both A. polyschistum and A. sessiliflorum need separate drug names.
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备注/Memo
基金项目：国家科技重大专项－重大新药创制（2014ZX09304- 307-001-019）
更新日期/Last Update:
2018-06-14