ZHANG Ping,MA Xiao,WANG Xiao-lin,et al.Study on HPLC Fingerprint of Different Proportions of Angelicae Pubescentis Radix in Angelicae Sinensis Radix[J].zhongguo zhongyiyao xinxi zazhi,2018,25(11):83-86.[doi:10.3969/j.issn.1005-5304.2018.11.018]
当归中混入不同比例独活HPLC特征指纹图谱研究
- Title:
- Study on HPLC Fingerprint of Different Proportions of Angelicae Pubescentis Radix in Angelicae Sinensis Radix
- 文章编号:
- 1005-5304(2018)11-0083-04
- 分类号:
- R284.1
- 文献标志码:
- A
- 摘要:
- 目的 建立测定当归中按不同比例掺入独活的HPLC分析方法及特征指纹图谱，为HPLC特征指纹图谱法甄别当归药材掺伪样品提供依据。方法 采用Agilent TC-C18色谱柱（5 μm，4.6 mm×250 mm），以乙腈（A）-0.05%磷酸（B）为流动相，梯度洗脱（0～20 min，95%～75%B；20～60 min，75%～65%B；60～80 min，65%～5%B），流速1 mL/min，检测波长254 nm，柱温30 ℃。采用《中药色谱指纹图谱相似度评价系统》对当归、独活及掺伪样品的HPLC图谱进行比较分析。结果 当归和独活色谱图中各色谱峰分离良好，HPLC指纹图谱中有11个峰可作为区别点，其中7号峰为阿魏酸，32号峰为蛇床子素，34号峰为二氢欧芹醇当归酸酯。结论 本方法快速、简单易行，能够全面反映当归药材品质，可以明确判断当归中是否混入独活。
- Abstract:
- Objective To establish HPLC analysis methods and fingerprint of different proportions of Angelicae Pubescentis Radix in Angelicae Sinensis Radix; To use HPLC fingerprint method to identify the adulteration of Angelicae Pubescentis Radix in Angelicae Sinensis Radix. Methods Agilent TC-C18 column (5 μm, 4.6 mm × 250 mm) was adopted. Acetonitrile (A) - 0.05% phosphoric acid (B) was the mobile phase, and the gradient elution (0–20 min, 95%–75%B; 20–60 min, 75% –65%B; 60–80 min, 65%–5%B) was used; The flowrate was 1 mL/min; Detection wavelength was 254 nm; Column temperature was 30 ℃. The HPLC spectra of Angelicae Sinensis Radix, Angelicae Pubescentis Radix and adulterated samples were compared and analyzed by using TCM chromatogram fingerprint similarity evaluation system. Results Angelicae Sinensis Radix and Angelicae Pubescentis Radix showed good chromatographic peaks in chromatogram separation . 11 peak s in HPLC fingerprint could be used as the mark, including 7th peak for ferulic acid, 32 th peak for osthole, and 34th peak for columbianadin. Conclusion This method is rapid, simple and feasible, can fully reflect the quality of Angelicae Sinensis Radix and clearly identify whether Angelicae Pubescentis Radix is adulterated in Angelicae Sinensis Radix.
参考文献/References:
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备注/Memo
基金项目：甘肃省食品药品科研项目（2017GSFDA012、2017GSFDA017、2018GSFDA008）；甘肃省科技重大专项（17ZDZFA009）
更新日期/Last Update:
2018-10-22