YANG Renyi,LIU Lijuan,KANG Lei,et al.Study on Mechanism of Huoxue Rongluo Prescription in Angiogenesis of Cerebral Infarction Based on Network Pharmacology[J].zhongguo zhongyiyao xinxi zazhi,2020,27(8):98-105.[doi:10.3969/j.issn.1005-5304.201907340]
基于网络药理学的活血荣络方对脑梗死血管新生作用机制研究
- Title:
- Study on Mechanism of Huoxue Rongluo Prescription in Angiogenesis of Cerebral Infarction Based on Network Pharmacology
- 文章编号:
- 1005-5304(2020)08-0098-08
- 分类号:
- R277.733.3；R285.5
- 文献标志码:
- A
- 摘要:
- 目的 采用网络药理学方法研究活血荣络方在脑梗死血管新生中的作用及其机制。方法 通过中药系统药理学数据库与分析平台（TCMSP）收集活血荣络方的主要活性成分并进行初步筛选，采用Swiss Target Prediction数据库预测主要活性成分靶点，通过GeneCards数据库预测脑梗死血管新生作用靶点，二者取交集得出共同靶点。采用String数据库及Cytoscape软件Network analyzer功能构建共同靶点蛋白相互作用网络。采用DAVID数据库结合Cytoscape软件对共同靶点进行GO及KEGG富集分析。结果 收集并筛选出活血荣络方422个活性成分、1028个靶点，脑梗死血管新生169个靶点，共同靶点42个，其中IL6、TNF、AKT1、CASP3相互作用最明显，主要调控氧化应激、细胞凋亡、蛋白磷酸化、内皮细胞及平滑肌细胞增殖等生物过程及HIF-1信号通路、癌症途径、TNF信号通路、能量代谢途径、PI3K-Akt信号通路、mTOR信号通路等。结论 活血荣络方在脑梗死血管新生中体现了多成分、多靶点、多途径的作用特点，有助于进一步诠释活血荣络方的药理学作用机制。
- Abstract:
- Objective To study the role and mechanism of Huoxue Rongluo Prescription in angiogenesis of cerebral infarction by network pharmacology. Methods The main active components of Huoxue Rongluo Prescription were collected and screened by Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). The Swiss Target Prediction database was used to predict the main active component targets, and the target of angiogenesis in cerebral infarction was predicted by GeneCards database, and the common targets were screened and integrated. A common target PPI network was constructed using String database and Cytoscape software Network analyzer function. GO and KEGG enrichment analysis were performed on the common target using DAVID database combined with Cytoscape software. Results Totally 422 active components of Huoxue Rongluo Prescription, 1028 targets, 169 targets for angiogenesis in cerebral infarction, and 42 common targets were screened, among which IL6, TNF, AKT1 and CASP3 were most obviously interacted, mainly regulating oxidative stress, apoptosis, protein phosphorylation, endothelial cells and smooth muscle cell proliferation and other biological processes , and HIF-1 signaling pathway, cancer pathway, TNF signaling pathway, energy metabolism pathway, PI3K-Akt signaling pathway, and mTOR signaling pathway. Conclusion Huoxue Rongluo Prescription demonstrates the characteristics of multi-component, multi-target and multi-pathway in angiogenesis of cerebral infarction, which is helpful to further interpret the pharmacological mechanism of Huoxue Rongluo Prescription.
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备注/Memo
基金项目：国家自然科学基金（81874463）；湖南省科技计划（2017SK4005）；湖南省中医药科研计划（201824、2020045）；湖南省财政中医药项目（rsk-010-13[006-09]）
更新日期/Last Update:
2020-07-20