YAO Dongsheng,XU Lin,LAN Tianying,et al.Effects of Shenshuai Decoction on Intestinal Permeability and Microinflammation in Rats with Renal Failure[J].zhongguo zhongyiyao xinxi zazhi,2021,28(1):67-72.[doi:10.19879/j.cnki.1005-5304.202007364]
肾衰Ⅱ号方对肾功能衰竭大鼠肠通透性及微炎症的影响
- Title:
- Effects of Shenshuai Decoction on Intestinal Permeability and Microinflammation in Rats with Renal Failure
- 文章编号:
- 1005-5304(2021)01-0067-06
- Keywords:
- chronic renal failure; Shenshuai Decoction; microinflammatory state; intestinal permeability ; rats
- 分类号:
- R285.5
- 文献标志码:
- A
- 摘要:
- 目的 观察肾衰Ⅱ号方对肾功能衰竭大鼠肠通透性及微炎症的影响，探讨其作用机制。方法 采用5/6肾切除法制备大鼠肾功能衰竭模型。40只SD雄性大鼠，随机分为假手术组、模型组、肾衰Ⅱ号方组和阳性对照组。各给药组给予相应药物，假手术组和模型组给予等量生理盐水，共60 d，处死前3.5 h均给予内毒素10 mg/kg灌胃。测试盒分析仪检测血清肌酐（Cr）、尿素氮（BUN）含量，HE染色观察肾脏、肠道病理变化，鳌试剂法检测下腔静脉血浆内毒素含量，ELISA检测相关炎症因子水平，免疫组化和RT-PCR分别检测肠紧密连接蛋白Claudin-1、Occludin蛋白和mRNA的表达。结果 与假手术组比较，模型组大鼠肾组织结构紊乱，Cr和BUN、内毒素及炎症因子水平明显升高（P＜0.05），Claudin-1、Occludin蛋白和mRNA表达明显降低（P＜0.05）；与模型组比较，肾衰Ⅱ号方组大鼠肾组织病理变化减轻，肾功能改善，内毒素及炎症因子水平明显降低（P＜0.05），Claudin-1、Occludin蛋白表达明显升高（P＜0.05），mRNA表达无明显变化。结论 肾衰Ⅱ号方可改善5/6肾切除大鼠肾功能水平，调整肠道通透性，减少肠源性内毒素入血，从而改善模型大鼠微炎症状态。
- Abstract:
- Objective To explore the effects of Shenshuai Ⅱ Decoction on intestinal permeability and micro- inflammation in rats with renal failure; To discuss its mechanism. Methods The 5/6 nephrectomy method was used to prepare rat renal failure models. 40 male SD rats were randomly divided into control group, sham-operation group, model group, Shenshuai Ⅱ Decoction group, and positive control group. Each administration group was given corresponding drugs, the sham-operation group and the model group were given the same amount of normal saline for 60 days, and endotoxin 10 mg/kg was given by gavage 3.5 hours before execution. Test kit analyzer was used to detect serum Cr and BUN content . HE staining was used to observe pathological changes of kidney and intestine . Chelol reagent method was used to detect plasma endotoxin content of inferior vena cava . ELISA was used to detect related inflammatory factors . Immunohistochemistry and RT-PCR were used to detect expressions of intestinal tight junction protein Claudin-1, Occludin protein and mRNA. Results Compared with the sham-operation group, the renal tissue structure of the model group was disordered, and the levels of Cr, BUN, endotoxin and inflammatory factors significantly increased (P<0.05), and Claudin-1, Occludin protein and mRNA expressions were significantly reduced (P<0.05). Compared with the model group, the pathological changes of renal tissue were reduced, the renal function was improved, the levels of endotoxin and inflammatory factors were reduced, Claudin-1, Occludin protein expressions significantly increased in the Shenshuai Ⅱ Decoction group ( P<0.05), and no significant change was found in mRNA expression. Conclusion Shenshuai Ⅱ Decoction can improve the renal function of 5/6 nephrectomy rats, and adjust intestinal permeability and reducing intestinal endotoxin, in order to improve the microinflammatory state of model rats.
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备注/Memo
基金项目：国家自然科学基金（81704015）；上海市进一步加快中医药事业发展三年行动计划2018年－2020年[ZY(2018－2020)-CCCX-2003-08，ZY(2018－2020)-FWTX-7005]；曙光医院四明基金（SGKJ-201808）；上海市浦东新区卫生系统重点学科群建设资助项目（PWZxq2017-07）
更新日期/Last Update:
2020-12-18