WANG Qingya,LI Fusheng,JIANG Xiaoyu,et al.Rapid Content Determination of Atractylenolides , , in Astractylodis Macrocephalae Rhizoma Based on Outer Product Fusion Spectra Combined with HPLC[J].zhongguo zhongyiyao xinxi zazhi,2021,28(1):86-91.[doi:10.19879/j.cnki.1005-5304.202004442]
外积融合光谱结合HPLC快速测定白术内酯Ⅰ、Ⅱ、Ⅲ含量
- Title:
- Rapid Content Determination of Atractylenolides , , in Astractylodis Macrocephalae Rhizoma Based on Outer Product Fusion Spectra Combined with HPLC
- 文章编号:
- 1005-5304(2021)01-0086-06
- Keywords:
- X-ray fluorescence spectroscopy; near infrared spectroscopy; N-way partial least square; atractylenolide; outer product fusion
- 分类号:
- R284.1
- 文献标志码:
- A
- 摘要:
- 目的 采用外积融合光谱结合HPLC建立白术内酯Ⅰ、Ⅱ、Ⅲ的定量分析模型，以实现快速检测。方法 收集不同产地白术饮片123批，采用HPLC测定白术内酯Ⅰ、Ⅱ、Ⅲ的含量，同时采集X射线荧光光谱和近红外光谱，采用多维偏最小二乘法建立3种白术内酯的定量分析模型。结果 白术中白术内酯Ⅰ、Ⅱ、Ⅲ的外积融合光谱定量分析模型的决定系数（R2）分别为0.946 4、0.917 2、0.955 8，校正均方根误差（RMSEC）分别为0.021 8、0.018 1、0.040 0，预测均方根误差（RMSEP）分别为0.031 1、0.025 8、0.049 8。结论 本研究建立的白术内酯Ⅰ、Ⅱ、Ⅲ外积融合光谱定量分析模型具有较好的准确性，可用于白术内酯Ⅰ、Ⅱ、Ⅲ的快速测定，为快速评价白术饮片质量提供依据。
- Abstract:
- Objective To establish a rapid quantification method for atractylenolides in Astractylodis Macrocephalae Rhizoma by outer product fusion (OPF) spectra and HPLC; To realize the rapid determination. Methods Totally 123 batches of Astractylodis Macrocephalae Rhizoma decoction pieces from different origins were collected. The contents of atractylenolide Ⅰ, Ⅱ and Ⅲ were determined by HPLC. X-ray fluorescence spectroscopy and near infrared spectroscopy of samples were also collected. The quantitative analysis model of three atractylenolides was established by N-way partial least square method. Results The determination coefficients (R2) of atractylenolide Ⅰ, Ⅱ and Ⅲ in OPF spectra analysis models were 0.946 4, 0.917 2, 0.955 8; root mean square error of calibration (RMSEC) were 0.021 8, 0.018 1, 0.040 0; root mean square error of prediction (RMSEP) were 0.031 1, 0.025 8, 0.049 8, respectively. Conclusion The established OPF quantitative analysis model of three types of atractylenolide in Astractylodis Macrocephalae Rhizoma has a good accuracy, which can be used for the rapid determination of atractylenolideⅠ, Ⅱ, and Ⅲ, providing a basis for the rapid evaluation of the quality of Astractylodis Macrocephalae Rhizoma decoction pieces.
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备注/Memo
基金项目：江西省中医药管理局项目（2020J010）；核技术应用教育部工程研究中心开放基金（HJSJYB2018-5）
更新日期/Last Update:
2020-12-18